Secretion of green fluorescent protein from recombinant baculovirus-infected insect cells.

@article{Laukkanen1996SecretionOG,
  title={Secretion of green fluorescent protein from recombinant baculovirus-infected insect cells.},
  author={M L Laukkanen and Christian Oker-Blom and Kari Kein{\"a}nen},
  journal={Biochemical and biophysical research communications},
  year={1996},
  volume={226 3},
  pages={755-61}
}
Trichoplusia ni (High Five) and Spodoptera frugiperda (Sf21) cells were engineered for expression of epitope (Flag)-tagged signal peptide-green fluorescent protein (GFP) fusions to examine the suitability of GFP as a secretory marker. The recombinant baculovirus-infected cells became fluorescent, and the High Five cells but not Sf21 cells secreted GFP in the culture medium as detected by the presence in the culture supernatant of a Flag-immunoreactive 30-kDa species and the characteristic 510… CONTINUE READING
8 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-8 of 8 extracted citations

Similar Papers

Loading similar papers…