Corpus ID: 35393886

Secretion of gamma-glutamyl hydrolase in vitro.

@article{OConnor1991SecretionOG,
  title={Secretion of gamma-glutamyl hydrolase in vitro.},
  author={B. O'Connor and R. Rotundo and Z. Nimec and J. McGuire and J. Galivan},
  journal={Cancer research},
  year={1991},
  volume={51 15},
  pages={
          3874-81
        }
}
gamma-Glutamyl hydrolase (also known as conjugase) is a ubiquitous enzyme that has the capacity to cleave folyl- and antifolylpolyglutamates. This study has revealed that the enzyme is secreted by primary cultures of rat hepatocytes and by H35 hepatoma cells. H35 cells have lower cellular levels of gamma-glutamyl hydrolase than do hepatocytes but secrete a greater proportion of gamma-glutamyl hydrolase. More than 99% of the total enzyme from H35 cells accumulated in the medium after 48 h. The… Expand
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