Secretion of functional antibody and Fab fragment from yeast cells.

  title={Secretion of functional antibody and Fab fragment from yeast cells.},
  author={Arnold H. Horwitz and C. P. Chang and Marc D. Better and Karl Erik Hellstrom and Randy R. Robinson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={85 22},
We have constructed yeast strains that secrete functional mouse-human chimeric antibody and its Fab fragment into the culture medium. For chimeric whole antibody, cDNA copies of the chimeric light-chain and heavy-chain genes of an anti-tumor antibody were inserted into vectors containing the yeast phosphoglycerate kinase promoter, invertase signal sequence, and phosphoglycerate kinase polyadenylylation signal. Simultaneous expression of these genes in yeast resulted in secretion of properly… 
Synthesis and self-assembly of a functional monoclonal antibody in transgenic Nicotiana tabacum
Synthesis of light and heavy chains and assembly to antibodies was detected in transgenic tobacco tissue using specific secondary antibodies and a significant amount of assembled antibodies was found within chloroplasts.
Expression and secretion of an assembled tetrameric CH2-deleted antibody in E. coli.
A functional assembled antibody variant that is secreted into the media in E. coli is a CH2-deleted chimeric antibody 14, which was previously shown to be a potentially useful reagent for radioimmunodetection of human tumors.
Cassette vectors for conversion of Fab fragments into full-length human IgG1 monoclonal antibodies by expression in stably transformed insect cells.
It is concluded that the cassette vectors designed for converting human Fab fragments selected from combinatorial phage display libraries into full-length IgG(1) monoclonal antibodies (MAbs) could serve as valuable tools for generating human IgG (1) antibodies.
Characterization of Humanized Antibodies Secreted by Aspergillus niger
No significant difference between the glycosylated antibody produced by Aspergillus and that produced by mammalian cell cultures was observed in tests for affinity, avidity, pharmacokinetics, or antibody-dependent cellular cytotoxicity function.
T‐Cell‐Targeted Immunofusion Proteins from E. coli
  • M. Better
  • Medicine, Biology
    Annals of the New York Academy of Sciences
  • 1996
This work constructed a family of immunofusion proteins linking the humanized Fab, F(ab')2, or single-chain antibody form of the H65 antibody with the plant ribosome-inactivating protein gelonin to maximize the product yield and simplify the production process.
Assembly of monoclonal antibodies with IgG1 and IgA heavy chain domains in transgenic tobacco plants
The results demonstrate that IgA as well as IgG class antibodies can be assembled correctly in tobacco plants and suggest that transgenic plants may be suitable for high‐level expression of more complex genetically engineered immunoglobulin molecules.
Production of Genetically Engineered Antibodies in Myeloma Cells: Design, Expression, and Applications
The criteria for producing recombinant antibodies in myeloma cells are discussed and immunoglobulin fusion proteins, in which antigen specificities have been fused to heterologous proteins, have been produced successfully.
Expression and detection of ScFvB9 and its mutant in recombinant phage antibody system.
The generated mutant ScFVB9-6 showed slightly higher antigen binding activity than native ScFvB9 as a soluble antibody in this RPAS, which was confirmed by enzyme-linked immunosorbent assay (ELISA) and Western blotting analysis.
Efficient expression of recombinant human monoclonal antibodies in Drosophila S2 cells.
The main advantages with this Drosophila S2 cell line for expression of Ig molecules isolated as Fab or scFv cDNA from phage-displayed libraries were its robustness and the much faster establishment of stable, high level producing cell lines.
Production of antibodies in transgenic plants
The results demonstrate that production of immunoglobulins and assembly of functional antibodies occurs very efficiently in tobacco and Assembly of subunits by sexual cross might be a generally applicable method for expression of heterologous multimers in plants.