Secretion of functional antibody and Fab fragment from yeast cells.

@article{Horwitz1988SecretionOF,
  title={Secretion of functional antibody and Fab fragment from yeast cells.},
  author={Arnold H. Horwitz and C. P. Chang and Marc D. Better and Karl Erik Hellstrom and Randy R. Robinson},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1988},
  volume={85 22},
  pages={
          8678-82
        }
}
We have constructed yeast strains that secrete functional mouse-human chimeric antibody and its Fab fragment into the culture medium. For chimeric whole antibody, cDNA copies of the chimeric light-chain and heavy-chain genes of an anti-tumor antibody were inserted into vectors containing the yeast phosphoglycerate kinase promoter, invertase signal sequence, and phosphoglycerate kinase polyadenylylation signal. Simultaneous expression of these genes in yeast resulted in secretion of properly… 
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