Secretion of endogenous and exogenous proteins from polarized MDCK cell monolayers.

  title={Secretion of endogenous and exogenous proteins from polarized MDCK cell monolayers.},
  author={T. Gottlieb and G. Beaudry and L. Rizzolo and A. Colman and M. Rindler and M. Adesnik and D. Sabatini},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={83 7},
Confluent monolayers of MDCK (Madin-Darby canine kidney) cells provide a widely used system to study the biogenesis of epithelial cell polarity. We now report that these cells are also capable of the vectorial constitutive secretion of a major endogenous product, a glycoprotein of 81 kDa, which is released into the medium from the apical surface within 30 min of its synthesis. This release represents a bona fide exocytotic secretory process and is not the result of proteolytic cleavage of a… Expand
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