Secretion of biologically active murine interleukin-2 by Lactococcus lactis subsp. lactis.

@article{Steidler1995SecretionOB,
  title={Secretion of biologically active murine interleukin-2 by Lactococcus lactis subsp. lactis.},
  author={Lothar Steidler and James M Wells and A. H. M. Raeymaekers and Jo{\"e}l Vandekerckhove and Walter Fiers and Erik Remaut},
  journal={Applied and environmental microbiology},
  year={1995},
  volume={61 4},
  pages={1627-9}
}
Secretion of functional recombinant murine interleukin-2 (mIL2) by Lactococcus lactis was achieved by fusion of the sequence encoding mature mIL2 to the secretion signal leader of the lactococcal usp45 gene placed under transcriptional control of the phage T7 promoter-T7 RNA polymerase expression system. The recombinant mature mIL2 was one of only a few proteins which accumulated in the growth medium. Sequence analysis revealed correct processing at the first amino acid of the mature protein. A… CONTINUE READING

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