Secretion of a foreign protein from budding yeasts is enhanced by cotranslational translocation and by suppression of vacuolar targeting


BACKGROUND Budding yeasts are often used to secrete foreign proteins, but the efficiency is variable. To identify roadblocks in the yeast secretory pathway, we used a monomeric superfolder GFP (msGFP) as a visual tracer in Saccharomyces cerevisiae and Pichia pastoris. RESULTS One roadblock for msGFP secretion is translocation into the ER. Foreign proteins… (More)
DOI: 10.1186/s12934-014-0125-0


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