Secretion of Cpn0796 from Chlamydia pneumoniae into the host cell cytoplasm by an autotransporter mechanism.

@article{Vandahl2005SecretionOC,
  title={Secretion of Cpn0796 from Chlamydia pneumoniae into the host cell cytoplasm by an autotransporter mechanism.},
  author={Brian Berg Vandahl and Allan Stensballe and Peter Roepstorff and G. Christiansen and Svend Birkelund},
  journal={Cellular microbiology},
  year={2005},
  volume={7 6},
  pages={825-36}
}
By comparison of proteome profiles of purified Chlamydia pneumoniae and whole lysates of C. pneumoniae infected HEp-2 cells, an N-terminal fragment of the previously uncharacterized chlamydial protein Cpn0796 was identified as a secreted protein. A 38 kDa cleavage product of Cpn0796 was present in infected cells, whereas only the 65 kDa full-length Cpn0796 could be detected in purified Chlamydia. Confocal immunofluorescence microscopy demonstrated that Cpn0796 was localized in the Chlamydia… CONTINUE READING