Secretion mechanism of Bacillus subtilis levansucrase: characterization of the second step.

@article{Chambert1988SecretionMO,
  title={Secretion mechanism of Bacillus subtilis levansucrase: characterization of the second step.},
  author={R{\'e}gis Chambert and Marie-Françoise Petit-Glatron},
  journal={Journal of general microbiology},
  year={1988},
  volume={134 5},
  pages={
          1205-14
        }
}
The kinetics of levansucrase secretion were examined in a strain of Bacillus subtilis which overproduces the enzyme (sacUh). Pulse-labelling experiments indicated that the second step of the levansucrase secretion process has the properties of a membrane active-transport system. This event appears to be directly linked to the influx of iron into the bacteria. The response of B. subtilis to the inducer of levansucrase synthesis was modulated by ferrichelators and the extent of the response… CONTINUE READING

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