Secretion cloning vectors in Escherichia coli.

  title={Secretion cloning vectors in Escherichia coli.},
  author={John Ghrayeb and Hiroshi Kimura and Masayasu Takahara and Hansen Hsiung and Yoshihiro Masui and M. Inouye},
  journal={The EMBO journal},
  volume={3 10},
The DNA fragment coding for the signal peptide of the OmpA protein, a major outer membrane protein of Escherichia coli, has been inserted into the high-level expression vectors, pIN-III. A foreign DNA fragment can be cloned in any one of the three reading frames at the unique EcoRI, HindIII or BamHI sites immediately after the ompA signal peptide coding sequence. The cloned foreign gene is under the control of both the lpp promoter and the lac promoter-operator. The expression of the gene is… CONTINUE READING