Secretion and overproduction of carboxypeptidase Y by a Saccharomyces cerevisiae ssl1 mutant strain.

@article{Ichikawa1993SecretionAO,
  title={Secretion and overproduction of carboxypeptidase Y by a Saccharomyces cerevisiae ssl1 mutant strain.},
  author={Kimihisa Ichikawa and Yoshinobu Shiba and Y. Jigami and Nobufusa Serizawa},
  journal={Bioscience, biotechnology, and biochemistry},
  year={1993},
  volume={57 10},
  pages={1686-90}
}
Carboxypeptidase Y (CPY; EC 3.4.16.1) is the yeast vacuolar protease. To have CPY secreted and to increase its secretion level, we tried to express the prepro-CPY gene under the control of the inducible GAL10 promoter or constitutive ENO1 promoter on a multicopy plasmid. In the strains KK4, PEP4, and A2-1-1A, carrying the CPY expression plasmid, active CPY was not detected in the culture broth although the CPY activity was greatly increased inside the cells. In contrast, when we used a strain… CONTINUE READING