Secretion and gene expression of inhibin, oxytocin and steroid hormones during the in vitro differentiation of bovine granulosa cells.

@article{Luck1990SecretionAG,
  title={Secretion and gene expression of inhibin, oxytocin and steroid hormones during the in vitro differentiation of bovine granulosa cells.},
  author={Martin R. Luck and Raymond J Rodgers and John K. Findlay},
  journal={Reproduction, fertility, and development},
  year={1990},
  volume={2 1},
  pages={
          11-25
        }
}
Bovine granulosa cells were cultured under defined conditions to examine (1) their secretion of immunoreactive inhibin, oxytocin, progesterone and oestradiol during differentiation in vitro; (2) their expression, by Northern analysis, of specific mRNAs for inhibin and oxytocin as compared with uncultured cells; (3) possible interrelationships between the four secreted hormones; and (4) the hypothesis that androgens and steroidogenesis influence the secretion of inhibin. The secretion of inhibin… 
The biphasic modulation of inhibin mRNA levels and secretion by PMSG in rat granulosa cells in vitro.
TLDR
It is demonstrated that low doses of follicle stimulating hormone/luteinizing hormone (FSH/LH)-like (PMSG) activity enhances and high doses decrease the steady-state mRNA levels of inhibin in rat granulosa cells in vitro; this biphasic regulation in vitro reflects the differential regulation of inhibitin secretion observed during the rat oestrous cycle.
Effect of FSH and cell localization on dimeric inhibin-A secretion from bovine granulosa cells in culture.
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It is concluded that FSH stimulates dimeric inhibin-A secretion from bovine granulosa cells, the 62 kDa form of inhib in-A may be more responsive to FSH than the 58 k da form, and the spatial differentiation ofgranulosa cell function within the follicle previously observed for oestradiol secretion was also observed.
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Structure of the alpha-inhibin gene and its regulation in the ruminant gonad: inverse relationship to oxytocin gene expression.
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Together the results indicate that a differentiation step occurring in Sertoli cells at early puberty and in granulosa cells at luteinization involves comparable regulation of genes through the sequential action of different cAMP-linked transcription factors.
Activin-A inhibits oxytocin and progesterone production by preovulatory bovine granulosa cells in vitro.
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Bovine activin-A has an autocrine action on bovine granulosa cells in vitro, to inhibit basal production of OT and progesterone, consistent with the role of activ in delaying the process of luteinization.
Low and high concentrations of gonadotropins differentially regulate hormone production by theca interna and granulosa cells from bovine preovulatory follicles.
TLDR
To address the question of how changes in gonadotropin concentrations in vivo regulate this follicular/luteal phase shift in hormone production, theca interna and granulosa cells were isolated from preovulatory follicles of Holstein heifers and cultured in defined medium containing insulin.
Acute effects of prostaglandin F2 alpha, luteinizing hormone, and estradiol on second messenger systems and on the secretion of oxytocin and progesterone from granulosa and early luteal cells of the ewe.
TLDR
Two granulosa and early luteal cells responded to LH treatment by an increase in progesterone output in a dose-responsive fashion and PGF2 alpha increased inositol phosphate accumulation in cells collected at both stages of the estrous cycle.
Oxytocin/neurophysin-I messenger ribonucleic acid in bovine granulosa cells increases after the luteinizing hormone (LH) surge and is stimulated by LH in vitro.
TLDR
The objective of this study was to determine whether the increase in OT secretion from granulosa cells after the LH surge is regulated at the level of mRNA accumulation, peptide synthesis, and/or peptide secretion.
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