Secreted antigens of the amastigote and promastigote forms of Leishmania infantum inducing a humoral response in humans and dogs.

@article{Cibrelus1999SecretedAO,
  title={Secreted antigens of the amastigote and promastigote forms of Leishmania infantum inducing a humoral response in humans and dogs.},
  author={P Cibrelus and Eric Pr{\'e}cigout and Denis Sereno and Bernard Carcy and J L Lemesre and Andr{\'e} Gorenflot},
  journal={Parasite},
  year={1999},
  volume={6 2},
  pages={121-9}
}
To study the antigens secreted by promastigote and amastigote forms of Leishmania infantum which are able to induce a humoral response in human patients and dogs, we have carried out immunoprecipitation assays with different supernatants of in vitro cultured parasites, metabolically labelled with [35S]methionine, using serum samples from human patients and dogs. In addition, some metabolic labelling experiments were performed daily during the in vitro culture parasite's life cycle to follow the… CONTINUE READING

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