Secondary structure and assembly mechanism of an oligomeric channel protein.

@article{Tobkes1985SecondarySA,
  title={Secondary structure and assembly mechanism of an oligomeric channel protein.},
  author={Nancy J Tobkes and B. A. Wallace and Hagan Bayley},
  journal={Biochemistry},
  year={1985},
  volume={24 8},
  pages={1915-20}
}
The alpha-toxin of Staphylococcus aureus is secreted as a water-soluble, monomeric polypeptide (Mr 33 182) that can assemble into an oligomeric membrane channel. By chemical cross-linking, we have confirmed that the major form of the channel is a hexamer. The circular dichroism spectrum of this hexamer in detergent revealed that it contains a high proportion of beta-sheet that we deduce must lie within the lipid bilayer when the protein is associated with membranes. The circular dichroism… CONTINUE READING