Second-order nonlinear optical microscopy of spider silk

@article{Zhao2017SecondorderNO,
  title={Second-order nonlinear optical microscopy of spider silk},
  author={Yue Zhao and Khuat Thi Thu Hien and Goro Mizutani and Harvey Rutt},
  journal={Applied Physics B},
  year={2017},
  volume={123},
  pages={1-8}
}
Asymmetric $$\upbeta $$β-sheet protein structures in spider silk should induce nonlinear optical interaction such as second harmonic generation (SHG) which is experimentally observed for a radial line and dragline spider silk using an imaging femtosecond laser SHG microscope. By comparing different spider silks, we found that the SHG signal correlates with the existence of the protein $$\upbeta $$β-sheets. Measurements of the polarization dependence of SHG from the dragline indicated that the… 
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14 Citations
Background Citations
1
Methods Citations
3

14 Citations

Observation of spider silk by femtosecond pulse laser second harmonic generation microscopy
An asymmetric β‐sheet structure of spider silk is said to induce optical second harmonic generation. In this paper, using an in‐house nonscanning type femtosecond pulse laser second harmonic…
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TLDR
It is found that the matrix effect of glycoprotein may cause ion suppression of water in aggregate glue droplets in orb‐weaver spiders, and after losing salts, water not only ceases to interact with glycoproteins but also oozes with the salts.
Mechanically Tunable Solid/Solid Phononic Crystals Through the Rearrangement of Hard Scatterers Controlled by the Deformation of Periodic Elastomeric Matrixes
The fixed band gap characteristic of passive phononic crystals (PCs) is possible to limit their applications in engineering. To overcome this shortcoming, inspired by the tunable mechanism of the…
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TLDR
This review summarizes the current state of the art on water collection by fibrous meshes and offers suggestions for the testing of new designs under laboratory conditions by classifying the parameters already reported in experimental set-ups.
Accumulation of cesium in glue balls of spiders' orb webs
Spiders capture prey by applying sticky coating substances to the spiral line of their orb webs. The main constituents of these sticky substances are glycoproteins and salts. This sticky substance is…
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References

SHOWING 1-10 OF 64 REFERENCES
Local Structure in Spider Dragline Silk Investigated by Two-Dimensional Spin-Diffusion Nuclear Magnetic Resonance†
TLDR
Two-dimensional (2D) spin-diffusion experiments show that the alanine-rich domains of the protein form β-sheet structures in agreement with one-dimensional NMR results from a different species of the genus Nephila but at variance with diffraction results.
Optical second-harmonic images of sacran megamolecule aggregates.
We have detected a second-order nonlinear optical response from aggregates of the ampholytic megamolecular polysaccharide sacran extracted from cyanobacterial biomaterials by using optical…
Image contrast immersion method for measuring refractive index applied to spider silks.
TLDR
The technique is used to obtain measurements of the refractive index of spider silks as a function of wavelength, for the first time, and an Abbe number for the radial silks of Plebs eburnus of ~32 is found.
The molecular structure of spider dragline silk: Folding and orientation of the protein backbone
TLDR
Two-dimensional solid-state NMR data indicate that all domains in dragline silk have a preferred secondary structure and are strongly oriented, with the chains predominantly parallel to the fiber.
Molecular Orientation and Two-Component Nature of the Crystalline Fraction of Spider Dragline Silk
TLDR
Solid-state 2H nuclear magnetic resonance data from unoriented, oriented, and supercontracted fibers suggest that it will be necessary to control the secondary structure of individual polymer molecules in order to obtain optimum properties in bio-inspired polymers.
Fiber Morphology of Spider Silk: The Effects of Tensile Deformation
The fiber morphology of the dragline silk of Nephila clavipes has been investigated by the detailed analysis of wide-angle X-ray diffraction (WAXD) patterns. WAXD gives the crystal lattice…
Molecular nanosprings in spider capture-silk threads
TLDR
Pulling on molecules in capture-silk fibres from Araneus has revealed rupture peaks due to sacrificial bonds, characteristic of other self-healing biomaterials.
Liquid crystals and flow elongation in a spider's silk production line
  • D. KnightF. Vollrath
  • Materials Science
    Proceedings of the Royal Society of London. Series B: Biological Sciences
  • 1999
Our observations on whole mounted major ampullate silk glands suggested that the thread is drawn from a hyperbolic die using a pre–orientated lyotropic liquid crystalline feedstock. Polarizing…
Structural studies of spider silk proteins in the fiber
TLDR
NMR and X‐ray diffraction data are consistent with the β‐sheets arising from the poly‐alanine sequences known to be present in the proteins of major ampullate silk as has been proposed previously.
The mechanical design of spider silks: from fibroin sequence to mechanical function.
TLDR
Comparison of MA silks from Araneus diadematus and Nephila clavipes shows variation in fibroin sequence and properties between spider species provides the opportunity to investigate the design of these remarkable biomaterials.
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