Second-order nonlinear optical microscopy of spider silk

@article{Zhao2017SecondorderNO,
  title={Second-order nonlinear optical microscopy of spider silk},
  author={Yue Zhao and Khuat Thi Thu Hien and Goro Mizutani and Harvey Rutt},
  journal={Applied Physics B},
  year={2017},
  volume={123},
  pages={1-8}
}
Asymmetric $$\upbeta $$β-sheet protein structures in spider silk should induce nonlinear optical interaction such as second harmonic generation (SHG) which is experimentally observed for a radial line and dragline spider silk using an imaging femtosecond laser SHG microscope. By comparing different spider silks, we found that the SHG signal correlates with the existence of the protein $$\upbeta $$β-sheets. Measurements of the polarization dependence of SHG from the dragline indicated that the… 
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15 Citations
Background Citations
2
Methods Citations
3

15 Citations

Observation of spider silk by femtosecond pulse laser second harmonic generation microscopy

An asymmetric β‐sheet structure of spider silk is said to induce optical second harmonic generation. In this paper, using an in‐house nonscanning type femtosecond pulse laser second harmonic…

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The pyriform silk molecular orientation of the attachment disc of a spider was studied using infrared-visible vibrational sum frequency generation (SFG) spectroscopy. When a spider secretes…

Sum frequency generation spectroscopy of the attachment disc of a spider.

Electric Field Effect on Optical Second-harmonic Generation of Amphoteric Megamolecule Aggregates

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Electric Field Effect on Optical Second-harmonic Generation of Amphoteric Megamolecule Aggregates.

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Interplay between Light and Functionalized Silk Fibroin and Applications

Active optics with silk

Abstract Optical devices have been traditionally fabricated using materials whose chemical and physical properties are finely tuned to perform a specific, single, and often static function, whereby…

Spectroscopic second and third harmonic generation microscopy using a femtosecond laser source in the third near-infrared (NIR-III) optical window.

TLDR
Second harmonic generation and third harmonic generation spectroscopic imaging were performed on biological samples using a femtosecond laser source in the third near-infrared optical window (NIR-III) to confirm that it can be utilized for various biological applications, including in vivo label-free imaging of living animals.

Recent Advances in Development of Functional Spider Silk-Based Hybrid Materials

TLDR
This review aims to provide a critical summary of up-to-date fabrication methods of spider silk-based organic-inorganic hybrid materials using both natural and bioengineered spider silk proteins blended with inorganic nanoparticles.

Analysis the water in aggregate glue droplets of spider orb web by TOF‐SIMS

TLDR
It is found that the matrix effect of glycoprotein may cause ion suppression of water in aggregate glue droplets in orb‐weaver spiders, and after losing salts, water not only ceases to interact with glycoproteins but also oozes with the salts.

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