Second-harmonic imaging of plant polysaccharides.

  title={Second-harmonic imaging of plant polysaccharides.},
  author={Guy C. Cox and Nuno Moreno and Jos{\'e} A Feij{\'o}},
  journal={Journal of biomedical optics},
  volume={10 2},
The application of second-harmonic generation (SHG) microscopy to plant materials has been neglected hitherto even though it would seem to have promise for identification and characterization of biologically and commercially important plant polysaccharides. We find that imaging of cellulose requires rather high laser powers, which are above optimal values for live cell imaging. Starch, however, is easily imaged by the technique at laser fluences compatible with extended cell viability. This… CONTINUE READING