Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms

  title={Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms},
  author={P. Campagnola and L. Loew},
  journal={Nature Biotechnology},
  • P. Campagnola, L. Loew
  • Published 2003
  • Materials Science, Medicine
  • Nature Biotechnology
  • Although the nonlinear optical effect known as second-harmonic generation (SHG) has been recognized since the earliest days of laser physics and was demonstrated through a microscope over 25 years ago, only in the past few years has it begun to emerge as a viable microscope imaging contrast mechanism for visualization of cell and tissue structure and function. Only small modifications are required to equip a standard laser-scanning two-photon microscope for second-harmonic imaging microscopy… CONTINUE READING
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