Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms

@article{Campagnola2003SecondharmonicIM,
  title={Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms},
  author={P. Campagnola and L. Loew},
  journal={Nature Biotechnology},
  year={2003},
  volume={21},
  pages={1356-1360}
}
Although the nonlinear optical effect known as second-harmonic generation (SHG) has been recognized since the earliest days of laser physics and was demonstrated through a microscope over 25 years ago, only in the past few years has it begun to emerge as a viable microscope imaging contrast mechanism for visualization of cell and tissue structure and function. Only small modifications are required to equip a standard laser-scanning two-photon microscope for second-harmonic imaging microscopy… Expand
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References

SHOWING 1-10 OF 31 REFERENCES
Second-harmonic imaging microscopy of living cells.
  • 237
  • PDF
Second harmonic imaging microscopy.
  • 58
High-resolution nonlinear optical imaging of live cells by second harmonic generation.
  • 463
  • PDF
Imaging cells and extracellular matrix in vivo by using second-harmonic generation and two-photon excited fluorescence
  • 812
  • PDF
Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation
  • 1,567
  • PDF
...
1
2
3
4
...