Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure

@article{Chen2012SecondHG,
  title={Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure},
  author={Xiyi Chen and Oleg Nadiarynkh and Sergey V. Plotnikov and Paul J Campagnola},
  journal={Nature Protocols},
  year={2012},
  volume={7},
  pages={654-669}
}
Second-harmonic generation (SHG) microscopy has emerged as a powerful modality for imaging fibrillar collagen in a diverse range of tissues. Because of its underlying physical origin, it is highly sensitive to the collagen fibril/fiber structure, and, importantly, to changes that occur in diseases such as cancer, fibrosis and connective tissue disorders. We discuss how SHG can be used to obtain more structural information on the assembly of collagen in tissues than is possible by other… CONTINUE READING

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