Second generation of pseudotype-based serum neutralization assay for Nipah virus antibodies: sensitive and high-throughput analysis utilizing secreted alkaline phosphatase.

@article{Kaku2012SecondGO,
  title={Second generation of pseudotype-based serum neutralization assay for Nipah virus antibodies: sensitive and high-throughput analysis utilizing secreted alkaline phosphatase.},
  author={Yoshihiro Kaku and Akira Noguchi and Glenn A Marsh and Jennifer Barr and Akiko Okutani and Kozue Hotta and Boldbaatar Bazartseren and Shuetsu Fukushi and Christopher C. Broder and Akio Yamada and Satoshi Inoue and Linfa Wang},
  journal={Journal of virological methods},
  year={2012},
  volume={179 1},
  pages={226-32}
}
Nipah virus (NiV), Paramyxoviridae, Henipavirus, is classified as a biosafety level (BSL) 4 pathogen, along with the closely related Hendra virus (HeV). A novel serum neutralization test was developed for measuring NiV neutralizing antibodies under BSL2 conditions using a recombinant vesicular stomatitis virus (VSV) expressing secreted alkaline phosphatase (SEAP) and pseudotyped with NiV F/G proteins (VSV-NiV-SEAP). A unique characteristic of this novel assay is the ability to obtain… CONTINUE READING