Seasonality affects post-thaw plasma membrane intactness and sperm velocities in spermatozoa from Thai AI swamp buffaloes (Bubalus bubalis).

@article{Koonjaenak2007SeasonalityAP,
  title={Seasonality affects post-thaw plasma membrane intactness and sperm velocities in spermatozoa from Thai AI swamp buffaloes (Bubalus bubalis).},
  author={Seri Koonjaenak and P Pongpeng and S Wirojwuthikul and Anders Johannisson and Annop Kunavongkrit and Heriberto Rodriguez-Martinez},
  journal={Theriogenology},
  year={2007},
  volume={67 9},
  pages={1424-35}
}
Altogether 218 frozen semen AI doses, prepared between 1980 and 1989 and also between 2003 and 2005 from 18 AI Thai swamp buffalo sires, were examined to determine whether seasonality affects post-thaw viability, as plasma membrane integrity (PMI, using SYBR-14/PI), plasma membrane stability (PMS, using Annexin-V/PI), or motility (Mot, using CASA). A thermoresistance test (38 degrees C for 60 min) was used to further analyze sperm survivability in vitro. All variables were compared over 3… CONTINUE READING

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