Seasonal change in the number of Cryptosporidium parvum oocysts in water samples from the rivers in Hokkaido, Japan, detected by the ferric sulfate flocculation method.

Abstract

An epidemiological study was carried out in natural water supplies of Hokkaido, one of the largest dairy prefectures in Japan. To investigate the prevalence of Cryptosporidium parvum (C. parvum) oocysts water samples were collected from three rivers in the eastern area of Hokkaido from August 1999 to October 2001, and C. parvum oocysts were collected and purified by the ferric sulfate flocculation method. The oocysts were detected using the immunofluorescent assay test (IFAT) and 4', 6-diamidino-2-phenylindole (DAPI) staining. The seasonal change in the number of oocysts detected was observed. Oocysts increased in numbers from the late summer to the early autumn (from August to November), thereafter, they exhibited a trend to decrease until December, when no oocysts could be detected. The maximum number of oocysts detected in the three rivers was 3.50, 5.00 and 3.33 oocysts/l, respectively. The oocyst density in river water changed in relation to the season in 1999, 2000 and 2001. This report first cleared up the seasonal changes in C. parvum oocysts number in river water.

Statistics

0100200'05'06'07'08'09'10'11'12'13'14'15'16'17
Citations per Year

245 Citations

Semantic Scholar estimates that this publication has 245 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Tsushima2003SeasonalCI, title={Seasonal change in the number of Cryptosporidium parvum oocysts in water samples from the rivers in Hokkaido, Japan, detected by the ferric sulfate flocculation method.}, author={Yoshinori Tsushima and Panagiotis Karanis and Takenori Kamada and Levi Hakwale Makala and Xuenan Xuan and Yukinobu Tohya and Hiroomi Akashi and Hideyuki Nagasawa}, journal={The Journal of veterinary medical science}, year={2003}, volume={65 1}, pages={121-3} }