Searching for allergens in maize kernels via proteomic tools.

  title={Searching for allergens in maize kernels via proteomic tools.},
  author={Elisa Fasoli and Elide Anna Pastorello and Laura Farioli and Joseph Scibilia and Giancarlo Aldini and Marina Carini and Adriano Marocco and Egisto Boschetti and Pier Giorgio Righetti},
  journal={Journal of proteomics},
  volume={72 3},
Up to the present, only one major allergen had been univocally identified by chemical analysis (N-terminal sequencing) in the salt-extractable (cytoplasmic) protein fraction of maize kernels (Zea mays): the lipid transfer protein (Pastorello et al., Allergy Clin. Immunol. 2000;106:744-751). In the present study, two-dimensional maps of kernel flour have been set up, the proteins transferred to nitrocellulose membranes and confronted with sera of various patients allergic to maize proteins. Via… CONTINUE READING