Seamless modification of wild-type induced pluripotent stem cells to the natural CCR5Δ32 mutation confers resistance to HIV infection.

@article{Ye2014SeamlessMO,
  title={Seamless modification of wild-type induced pluripotent stem cells to the natural CCR5Δ32 mutation confers resistance to HIV infection.},
  author={Lin Ye and Jiaming Wang and Ashley I Beyer and Fernando Teque and Thomas J. Cradick and Zhongxia Qi and Judy Chia-Chi Chang and Gang Bao and Marcus O Muench and Jingwei Yu and Jay A. Levy and Yuet Wai Kan},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2014},
  volume={111 26},
  pages={9591-6}
}
Individuals homozygous for the C-C chemokine receptor type 5 gene with 32-bp deletions (CCR5Δ32) are resistant to HIV-1 infection. In this study, we generated induced pluripotent stem cells (iPSCs) homozygous for the naturally occurring CCR5Δ32 mutation through genome editing of wild-type iPSCs using a combination of transcription activator-like effector nucleases (TALENs) or RNA-guided clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 together with the piggyBac technology… CONTINUE READING
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