Sea urchin egg preparations as systems for the study of calcium-triggered exocytosis.

@article{Zimmerberg1999SeaUE,
  title={Sea urchin egg preparations as systems for the study of calcium-triggered exocytosis.},
  author={Joshua Zimmerberg and Jens R Coorssen and Steven S. Vogel and Paul S. Blank},
  journal={The Journal of physiology},
  year={1999},
  volume={520 Pt 1},
  pages={15-21}
}
This paper reviews recent work in our laboratory on the mechanism of calcium-triggered exocytosis. Upon echinoderm egg fertilization, cortical secretory vesicle exocytosis is massive and synchronous. By combining physiological and molecular analyses with a variety of purified membrane isolates containing secretory vesicles that fuse to the plasma membrane or each other, we have characterized the final steps of this calcium-triggered exocytosis. Our kinetic analysis led to a functional… CONTINUE READING
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