Screening of sulfate-reducing bacteria in colonoscopy samples from healthy and colitic human gut mucosa.

@article{Zinkevich2000ScreeningOS,
  title={Screening of sulfate-reducing bacteria in colonoscopy samples from healthy and colitic human gut mucosa.},
  author={Zinkevich and Beech},
  journal={FEMS microbiology ecology},
  year={2000},
  volume={34 2},
  pages={
          147-155
        }
}
A PCR-based approach combined with microbiological cultivation methods was employed to determine the occurrence of sulfate-reducing bacteria (SRB) in colon biopsy samples from ulcerative colitis patients and from non-colitic controls. The detection of mucosa-associated SRB was carried out by digoxigenin-dUTP-labelled PCR amplification, in liquid Postgate medium B and in a new liquid medium, termed VM medium I. Using Postgate medium B, the growth of SRB was confirmed in 92% of the colitic… CONTINUE READING
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