Screening of specific antigens for SARS clinical diagnosis using a protein microarray.

@article{Lu2005ScreeningOS,
  title={Screening of specific antigens for SARS clinical diagnosis using a protein microarray.},
  author={Dan-dan Lu and Suhong Chen and Shi-meng Zhang and Min-li Zhang and Wei Zhang and Xiaochen Bo and Shengqi Wang},
  journal={The Analyst},
  year={2005},
  volume={130 4},
  pages={474-82}
}
In this study several SARS-CoV structural proteins and fragments were expressed in E. coli as GST or TRX fusion proteins. They were fabricated on a microarray and tested with sera from SARS patients. Antigenic screening indicated that recombinant GST-N2 fusion protein, the carboxy-terminus 213aa-423aa of N protein, was strongest positive and weakest non-specific compared with others. An indirect antibody ELISA method was developed and clinical positive and negative sera for their antibodies… CONTINUE READING