Screening of optimal cellulases from symbiotic protists of termites through expression in the secretory pathway of Saccharomyces cerevisiae.

Abstract

For direct and efficient ethanol production from cellulosic materials, we screened optimal cellulases from symbiotic protists of termites through heterologous expression with Saccharomyces cerevisiae. 11 cellulases, belonging to glycoside hydrolase families 5, 7, and 45 endoglucanases (EGs), were confirmed to produce with S. cerevisiae for the first time. A recombinant yeast expressing SM2042B24 EG I was more efficient at degrading carboxylmethyl cellulose than was Trichoderma reesei EG I, a major EG with high cellulolytic activity.

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@article{Todaka2011ScreeningOO, title={Screening of optimal cellulases from symbiotic protists of termites through expression in the secretory pathway of Saccharomyces cerevisiae.}, author={Nemuri Todaka and Risa Nakamura and Sigeharu Moriya and Moriya Ohkuma and Toshiaki Kudo and Haruo Takahashi and Nobuhiro Ishida}, journal={Bioscience, biotechnology, and biochemistry}, year={2011}, volume={75 11}, pages={2260-3} }