Screening of new antioxidant molecules using flow cytometry.

  title={Screening of new antioxidant molecules using flow cytometry.},
  author={Serge Ostrovidov and Patricia Franck and David O Joseph and Laurent Martarello and Gilbert Kirsch and Francine Belleville and Pierre Nabet and Brigitte Dousset},
  journal={Journal of medicinal chemistry},
  volume={43 9},
We present a flow cytometry technique to evaluate the antioxidative properties of molecules on living cells, using a stable murine-murine hybridoma (Mark 3) cell line routinely cultured. Using this technique, intracellular superoxide anions and peroxides were evaluated with dihydrorhodamine (DHR-123) and dichlorofluorescein diacetate (DCFH-DA), respectively. When cells were first incubated for 10 min with either H(2)O(2) or the xanthine (X)/xanthine oxidase (XO) system, this flow cytometric… CONTINUE READING
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