Screening of cDNA libraries on glass slide microarrays.

  title={Screening of cDNA libraries on glass slide microarrays.},
  author={Dave K Berger and Bridget Genevieve Crampton and Ingo Hein and Wiesner Vos},
  journal={Methods in molecular biology},
A quantitative screening method was developed to evaluate the quality of cDNA libraries constructed by suppression subtraction hybridization (SSH) or other enrichment techniques. The SSH technique was adapted to facilitate screening of the resultant library on a small number of glass slide microarrays. A simple data analysis pipeline named SSHscreen using "linear models for microarray data" (limma) functions in the R computing environment was developed to identify clones in the cDNA libraries… CONTINUE READING