Screening of HIV-1 Protease Using a Combination of an Ultra-High-Throughput Fluorescent-Based Assay and RapidFire Mass Spectrometry.

Abstract

HIV-1 protease (PR) represents one of the primary targets for developing antiviral agents for the treatment of HIV-infected patients. To identify novel PR inhibitors, a label-free, high-throughput mass spectrometry (HTMS) assay was developed using the RapidFire platform and applied as an orthogonal assay to confirm hits identified in a fluorescence… (More)
DOI: 10.1177/1087057115570838

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Cite this paper

@article{Meng2015ScreeningOH, title={Screening of HIV-1 Protease Using a Combination of an Ultra-High-Throughput Fluorescent-Based Assay and RapidFire Mass Spectrometry.}, author={Juncai Meng and Ming-tain Lai and Vandna Munshi and Jay Grobler and John McCauley and Paul D Zuck and Eric N. Johnson and Victor N. Uebele and Jeffrey D Hermes and Gregory C. Adam}, journal={Journal of biomolecular screening}, year={2015}, volume={20 5}, pages={606-15} }