Screening of DNA aptamers against myoglobin using a positive and negative selection units integrated microfluidic chip and its biosensing application.

@article{Wang2014ScreeningOD,
  title={Screening of DNA aptamers against myoglobin using a positive and negative selection units integrated microfluidic chip and its biosensing application.},
  author={Qing Wang and Wei Liu and Yuqian Xing and Xiaohai Yang and Kemin Wang and Rui Jiang and Pei Wang and Qing Zhao},
  journal={Analytical chemistry},
  year={2014},
  volume={86 13},
  pages={6572-9}
}
An aptamer screening method using a positive and negative selection units integrated microfluidic chip was introduced. Here, myoglobin (Myo), one of the early markers to increase after acute myocardial infarction, was used as the model. After 7-round selection, the aptamers, which exhibited dissociation constants (K(d)) in the nanomolar range (from 4.93 to 6.38 nM), were successfully obtained using a positive and negative selection units integrated microfluidic chip. The aptamer with the… CONTINUE READING
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