Screening ligands for membrane protein receptors by total internal reflection fluorescence: the 5-HT3 serotonin receptor.

@article{Schmid1998ScreeningLF,
  title={Screening ligands for membrane protein receptors by total internal reflection fluorescence: the 5-HT3 serotonin receptor.},
  author={Evelyne L Schmid and A. Tairi and Rund Hovius and Horst Vogel},
  journal={Analytical chemistry},
  year={1998},
  volume={70 7},
  pages={1331-8}
}
The screening of ligands for membrane receptor proteins is central to the discovery of new pharmaceutical drugs. We present a general method to reversibly attach receptor proteins via an affinity tag to a quartz surface and subsequently detect with high sensitivity the real-time binding of ligands by total internal reflection fluorescence. A serotonin-gated ion channel protein was immobilized, and the binding of a fluorescent ligand was investigated. The affinity and the kinetic parameters of… CONTINUE READING

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