Screening libraries to identify proteins with desired binding activities using a split-GFP reassembly assay.

@article{Jackrel2010ScreeningLT,
  title={Screening libraries to identify proteins with desired binding activities using a split-GFP reassembly assay.},
  author={Meredith E Jackrel and Aitziber L. Cortajarena and Tina Y Liu and Lynne Regan},
  journal={ACS chemical biology},
  year={2010},
  volume={5 6},
  pages={553-62}
}
Designer protein modules, which bind specifically to a desired target, have numerous potential applications. One approach to creating such proteins is to construct and screen libraries. Here we present a detailed description of using a split-GFP reassembly assay to screen libraries and identify proteins with novel binding properties. Attractive features of the split-GFP based screen are the absence of false positives and the simplicity, robustness, and ease of automation of the screen. Here, we… CONTINUE READING

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Ligand binding by TPR domains.

Protein science : a publication of the Protein Society • 2006

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