Screening for the presence of polyglycosylceramides in various tissues: partial characterization of blood group-active complex glycosphingolipids of rabbit and dog small intestines

@article{Podraza1997ScreeningFT,
  title={Screening for the presence of polyglycosylceramides in various tissues: partial characterization of blood group-active complex glycosphingolipids of rabbit and dog small intestines},
  author={Halina Miller Podraza and Gunnar Stenhagen and Thomas Larsson and Carita Andersson and K Karlsson},
  journal={Glycoconjugate Journal},
  year={1997},
  volume={14},
  pages={231-239}
}
Twenty different human and animal tissues were investigated for the presence of polyglycosylceramides. The glycolipids were isolated by peracetylation of dry tissue residues left after conventional lipid extraction, followed by extraction with chloroform and subsequent Sephadex LH-20, Sephadex LH-60 and silica gel chromatography. In most of the cases only trace amounts of complex glycolipids were found. Distinct bands of glycosphingolipids migrating on TLC plates in a region of brain… CONTINUE READING

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