Screening for mutations by enzyme mismatch cleavage with T4 endonuclease VII.

@article{Youil1995ScreeningFM,
  title={Screening for mutations by enzyme mismatch cleavage with T4 endonuclease VII.},
  author={R. Youil and B{\"o}rries Kemper and Richard G. H. Cotton},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1995},
  volume={92 1},
  pages={87-91}
}
Each of four possible sets of mismatches (G.A/C.T, C.C/G.G, A.A/T.T, and C.A/G.T) containing the 8 possible single-base-pair mismatches derived from isolated mutations were examined to test the ability of T4 endonuclease VII to consistently detect mismatches in heteroduplexes. At least two examples of each set of mismatches were studied for cleavage in the complementary pairs of heteroduplexes formed between normal and mutant DNA. Four deletion mutations were also included in this study. The… CONTINUE READING

From This Paper

Figures, tables, and topics from this paper.

Citations

Publications citing this paper.
Showing 1-10 of 46 extracted citations

Similar Papers

Loading similar papers…