Screening for intron-length polymorphisms in penaeid shrimps using exon-primed intron-crossing (EPIC)-PCR.

@article{Bierne2000ScreeningFI,
  title={Screening for intron-length polymorphisms in penaeid shrimps using exon-primed intron-crossing (EPIC)-PCR.},
  author={Nicolas Bierne and Sigrid A. Lehnert and Edouard B{\'e}dier and François Bonhomme and Stephen Stewart Moore},
  journal={Molecular ecology},
  year={2000},
  volume={9 2},
  pages={233-5}
}
Intron polymorphisms have been shown to yield substantial variability and were successfully used in several population genetics surveys (Lessa 1992; Côrte-Real et al . 1994; Daguin & Borsa 1999). Most studies to date have been restricted to a few genes and, frequently, polymorphic introns have been found by chance. To amplify introns, polymerase chain reaction (PCR) primers can be designed in flanking exons. This approach, called exon-primed intron-crossing (EPIC)-PCR (Palumbi 1995), has… CONTINUE READING