Screening for in vivo protein-protein interactions.


We describe an in vivo approach for the isolation of proteins interacting with a protein of interest. The protein of interest is "tagged" with a portion of the biotin carboxylase carrier protein (BCCP), encoded on a specially constructed plasmid, so that it becomes biotinylated in vivo. The "query" proteins (e.g., those in a cDNA library) are tagged by… (More)


Cite this paper

@article{Germino1993ScreeningFI, title={Screening for in vivo protein-protein interactions.}, author={F Joseph Germino and Zhi-xin Wang and Sherman Weissman}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={1993}, volume={90 3}, pages={933-7} }