Screening for cytokine mRNA in human villous and extravillous trophoblasts using the reverse-transcriptase polymerase chain reaction (RT-PCR).

@article{King1995ScreeningFC,
  title={Screening for cytokine mRNA in human villous and extravillous trophoblasts using the reverse-transcriptase polymerase chain reaction (RT-PCR).},
  author={Adam Philip King and Percy P Jokhi and Stephen K. Smith and Andrew M Sharkey and Yung Way Loke},
  journal={Cytokine},
  year={1995},
  volume={7 4},
  pages={364-71}
}
During the process of placental implantation, sessile villous trophoblast cells migrate from the villi into the decidua as isolated motile extravillous trophoblast cells. There is differential expression of the epidermal growth factor-receptor (EGF-R) and c-erbB2 proteins on villous and extravillous trophoblast populations. Using monoclonal antibodies to EGF-R and c-erbB2, we have obtained highly purified populations of villous and extravillous trophoblast by fluorescence activated cell sorting… CONTINUE READING
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