Screening for congenital disorders of glycosylation (CDG): transferrin HPLC versus isoelectric focusing (IEF).

@article{Quintana2009ScreeningFC,
  title={Screening for congenital disorders of glycosylation (CDG): transferrin HPLC versus isoelectric focusing (IEF).},
  author={E. Murias Quintana and Aleix Navarro-Sastre and Jos{\'e} Mar{\'i}a Hern{\'a}ndez-P{\'e}rez and Judit Garc{\'i}a-Villoria and R. Alejandra Montero and Rafael Artuch and A Garc{\'i}a Ribes and Paz Briones},
  journal={Clinical biochemistry},
  year={2009},
  volume={42 4-5},
  pages={408-15}
}
OBJECTIVES Transferrin isoelectrofocusing (Tf-IEF) is widely used to screen for Congenital Disorders of Glycosylation (CDG), but it is laborious, time-consuming, and not suitable for automation or accurate quantification. We present our experience and advantages of the implementation of Tf-HPLC. METHODS Sera were iron saturated, lipid precipitated and filtrated on Microcon-YM10. Glycoforms were separated by HPLC on a SOURCE 15Q anion-exchange column. Detection was at 470 nm. RESULTS We… CONTINUE READING

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