Screening for antiviral inhibitors of the HIV integrase-LEDGF/p75 interaction using the AlphaScreen luminescent proximity assay.

@article{Hou2008ScreeningFA,
  title={Screening for antiviral inhibitors of the HIV integrase-LEDGF/p75 interaction using the AlphaScreen luminescent proximity assay.},
  author={Yan Hou and Debra Mcguinness and Andrew J. Prongay and Boris Feld and Paul Ingravallo and Robert A. Ogert and Charles A. Lunn and John A. Howe},
  journal={Journal of biomolecular screening},
  year={2008},
  volume={13 5},
  pages={406-14}
}
Small-molecule inhibitors of HIV integrase (HIV IN) have emerged as a promising new class of antivirals for the treatment of HIV/AIDS. The compounds currently approved or in clinical development specifically target HIV DNA integration and were identified using strand-transfer assays targeting the HIV IN/viral DNA complex. The authors have developed a second biochemical assay for identification of HIV integrase inhibitors, targeting the interaction between HIV IN and the cellular cofactor LEDGF… CONTINUE READING