Screening for and analysis of methylation differences using methylation-sensitive single-strand conformation analysis.

@article{Dobrovic2002ScreeningFA,
  title={Screening for and analysis of methylation differences using methylation-sensitive single-strand conformation analysis.},
  author={Alexander Dobrovic and Tina Bianco and Lor Wai Tan and Tanya Sanders and Damian Hussey},
  journal={Methods},
  year={2002},
  volume={27 2},
  pages={134-8}
}
Methylation-sensitive single-strand conformation analysis (MS-SSCA) is a method of screening for methylation changes at CpG sites in a region of DNA. After bisulfite modification, the region of interest is amplified using primers specific for bisulfite-modified sequences. The amplified products are denatured and run on a nondenaturing polyacrylamide gel. The sequence differences caused by methylation lead to the formation of different secondary structures (conformers) with different mobilities… CONTINUE READING

From This Paper

Topics from this paper.

Similar Papers

Loading similar papers…