Screening factors effecting a response in soluble protein expression: formalized approach using design of experiments.

@article{Swalley2006ScreeningFE,
  title={Screening factors effecting a response in soluble protein expression: formalized approach using design of experiments.},
  author={Susanne E Swalley and John R Fulghum and Stephen P. Chambers},
  journal={Analytical biochemistry},
  year={2006},
  volume={351 1},
  pages={122-7}
}
We have integrated high-throughput expression and purification with quantitative protein analysis to identify factors influencing protein production. Application of high-throughput expression and purification, combined with automated gel capillary electrophoresis, allowed the quantitative analysis of multiple expression variables in a single experiment. An experimental design utilizing multiple factorial screens was employed to identify single factors and interactions having a significant… CONTINUE READING

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