Screening botanical extracts for quinoid metabolites.

  title={Screening botanical extracts for quinoid metabolites.},
  author={Benjamin M. Johnson and Judy L Bolton and Richard B van Breemen},
  journal={Chemical research in toxicology},
  volume={14 11},
Botanical dietary supplements represent a significant share of the growing market for alternative medicine in the USA, where current regulations do not require assessment of their safety. To help ensure the safety of such products, an in vitro assay using pulsed ultrafiltration and LC-MS-MS has been developed to screen botanical extracts for the formation of electrophilic and potentially toxic quinoid species upon bioactivation by hepatic cytochromes P450. Rat liver microsomes were trapped in a… CONTINUE READING