• Corpus ID: 88252126

Screening and identification of B cell antigenic epitopes of porcine epidemic diarrhea virus spike glycoprotein by phage display

@article{Sun2007ScreeningAI,
  title={Screening and identification of B cell antigenic epitopes of porcine epidemic diarrhea virus spike glycoprotein by phage display},
  author={Donghong Sun and Hongwu Lang and Hongyan Shi and Jianfei Chen and Xiaochen Cui and Cheng Bao Wang and You En Tong and Li Feng},
  journal={Progress in Biochemistry and Biophysics},
  year={2007},
  volume={34}
}
S1 gene targeted libraries containing the major immunodominant region(1~2 367 bp) of PEDV spike glycoprotein were constructed by phage-display vectors based on filamentous phage strain fd-tet,in which the exogenous polypeptides were expressed in the N-terminal of gene Ⅲ coat protein.The S1 libraries were panned three times using the purified rabbit sera against PEDV.Three peptides,displayed on recombinant phages,showed strong binding affinity with the PEDV antisera,and were designated as S1P1… 
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