Screening and evaluation of inhibitors of Plasmodium falciparum merozoite egress and invasion using cytometry.

@article{Bouillon2013ScreeningAE,
  title={Screening and evaluation of inhibitors of Plasmodium falciparum merozoite egress and invasion using cytometry.},
  author={Anthony Bouillon and Olivier Gorgette and Odile Mercereau-Puijalon and Jean-Christophe Barale},
  journal={Methods in molecular biology},
  year={2013},
  volume={923},
  pages={523-34}
}
Drug discovery programs heavily rely on assays adequately monitoring the activity of the drug on the -parasite stage targeted. So far, assays used to screen molecules active against Plasmodium falciparum parasites have mostly been based on measuring growth inhibition of asexual blood stages. We have developed a robust protocol allowing for monitoring parasite egress at the late schizont stage and subsequent erythrocyte invasion. This cytometry-based methodology uses nucleic acid labelling by… CONTINUE READING