Scl 70 autoantibodies from scleroderma patients recognize a 95 kDa protein identified as DNA topoisomerase I

@article{Guldner2004Scl7A,
  title={Scl 70 autoantibodies from scleroderma patients recognize a 95 kDa protein identified as DNA topoisomerase I},
  author={H. H. Guldner and Carin Szostecki and Hans Peter Vosberg and H. J. Lakomek and Edward Penner and Friedlinde A. Bautz},
  journal={Chromosoma},
  year={2004},
  volume={94},
  pages={132-138}
}
Sera of patients suffering from the autoimmune disease progressive systemic sclerosis (PSS) are known to contain autoantibodies which have been reported to recognize a 70 kDa antigenic protein, designated the Scl 70 antigen. By immunoblotting of nuclear extracts from HeLa cells with sera from scleroderma patients we observed that the size of the antigen present in such cells depends on the conditions of antigen isolation. When protease inhibitors were included in the extraction buffer, a 95 kDa… 
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