Schwann cell-enriched cultures from adult human peripheral nerve: a technique combining short enzymatic dissociation and treatment with cytosine arabinoside (Ara-C).

@article{CaldernMartnez2002SchwannCC,
  title={Schwann cell-enriched cultures from adult human peripheral nerve: a technique combining short enzymatic dissociation and treatment with cytosine arabinoside (Ara-C).},
  author={Diany Calder{\'o}n-Mart{\'i}nez and Zayra V. Garavito and Clara Spinel and Hern{\'a}n Hurtado},
  journal={Journal of neuroscience methods},
  year={2002},
  volume={114 1},
  pages={1-8}
}
Attempts to design the nerve cellular prostheses have focused on the production of autologous Schwann cells expanded in vitro as the essential component in the regeneration process of injured peripheral nerves. To obtain human Schwann cells of high quality we tested a short enzymatic dissociation protocol that optimized cellular viability levels. We also assessed patterns of bromodeoxyuridine (BrdU) incorporation in both Schwann cells and fibroblasts in the presence or absence of the… CONTINUE READING

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