Schmallenberg virus detection in bovine semen after experimental infection of bulls

@inproceedings{Poel2014SchmallenbergVD,
  title={Schmallenberg virus detection in bovine semen after experimental infection of bulls},
  author={Wilhelmus HM Van der Poel and Joyce M Parlevliet and Elisabeth R.A.M. Verstraten and Engbert A. Kooi and Renate Hakze-Van der Honing and Norbert Stockhofe},
  booktitle={Epidemiology and infection},
  year={2014}
}
To study Schmallenberg virus (SBV) excretion in bovine semen after experimental infection, two bulls were inoculated subcutaneously with a SBV isolate (1 ml Vero cell culture 106 TCID50). After inoculation (at day 0), semen was collected daily from both animals for 21 days and samples were tested for SBV by qRT-PCR assay. At 24 days post-inoculation both animals were subjected to necropsy and the genital organs and lymph nodes draining these organs were also tested for SBV RNA (qRT-PCR). After… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 11 extracted citations

Review: Risks of disease transmission through semen in cattle.

Animal : an international journal of animal bioscience • 2018
View 2 Excerpts

Preliminary serological evidence for Schmallenberg virus infection in China

Tropical Animal Health and Production • 2017
View 1 Excerpt

European interlaboratory comparison of Schmallenberg virus (SBV) real-time RT-PCR detection in experimental and field samples: The method of extraction is critical for SBV RNA detection in semen.

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc • 2015
View 9 Excerpts

References

Publications referenced by this paper.
Showing 1-10 of 13 references

Development of a virus neutralisation test to detect antibodies against Schmallenberg virus and serological results in suspect and infected herds

W Loeffen
Acta Veterinaria Scandinavia • 2012

Diagnostics for Schmallenberg virus

Van der Poel WHM
Veterinary Record 2012; • 2012
View 1 Excerpt

Schmallenberg virus –Europe (77): (NL, FR) virus

ProMED mail
RNA in bovine semen, • 2012
View 1 Excerpt

Similar Papers

Loading similar papers…