Schmallenberg virus detection in bovine semen after experimental infection of bulls

  title={Schmallenberg virus detection in bovine semen after experimental infection of bulls},
  author={Wilhelmus HM Van der Poel and Joyce M Parlevliet and Elisabeth R.A.M. Verstraten and Engbert A. Kooi and Renate Hakze-Van der Honing and Norbert Stockhofe},
  booktitle={Epidemiology and infection},
To study Schmallenberg virus (SBV) excretion in bovine semen after experimental infection, two bulls were inoculated subcutaneously with a SBV isolate (1 ml Vero cell culture 106 TCID50). After inoculation (at day 0), semen was collected daily from both animals for 21 days and samples were tested for SBV by qRT-PCR assay. At 24 days post-inoculation both animals were subjected to necropsy and the genital organs and lymph nodes draining these organs were also tested for SBV RNA (qRT-PCR). After… CONTINUE READING


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