Schemes of metabolic patterns of anabolic androgenic steroids for the estimation of metabolites of designer steroids in human urine

@article{Fragkaki2009SchemesOM,
  title={Schemes of metabolic patterns of anabolic androgenic steroids for the estimation of metabolites of designer steroids in human urine},
  author={Argyro G Fragkaki and Yiannis S Angelis and Anna Tsantili-Kakoulidou and Michael A. Koupparis and Constantinos D Georgakopoulos},
  journal={The Journal of Steroid Biochemistry and Molecular Biology},
  year={2009},
  volume={115},
  pages={44-61}
}
Unified metabolism schemes of anabolic androgenic steroids (AAS) in human urine based on structure classification of parent molecules are presented in this paper. Principal components analysis (PCA) was applied to AAS molecules referred in the World Anti-Doping Agency (WADA) list of prohibited substances, resulting to their classification into six distinct groups related to structure features where metabolic alterations usually occur. The metabolites of the steroids participating to these six… Expand
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In Vitro Synthesis of Metabolites of three Anabolic Androgenic Steroids, by Human Liver Microsomes
Anabolic androgenic steroids are substances frequently misused to improve physical performance in sports. To reveal substances misused as doping, athlete urine samples are collected and tested. To… Expand
ANALYTICAL METHODS FOR THE DETECTION OF NON- LABELED ANABOLIC ANDROGENIC STEROIDS IN FOOD SUPPLEMENTS
Recent studies showed that non-hormonal supplements such as vitamins, minerals and amino acids or other food additives can contain anabolic androgenic steroids not declared on the labels of the… Expand
Urinary detection of conjugated and unconjugated anabolic steroids by dilute-and-shoot liquid chromatography-high resolution mass spectrometry.
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A comprehensive detection of anabolic androgenic steroids metabolites is described using a 2-fold dilution of urine and subsequent analysis by liquid chromatography-high resolution mass spectrometry (LC-HRMS). Expand
Detection of designer steroid methylstenbolone in “nutritional supplement” using gas chromatography and tandem mass spectrometry: Elucidation of its urinary metabolites
TLDR
A human excretion study was carried out with four volunteers after a single oral dose of a new anabolic steroid known as methylstenbolone to determine the urinary metabolites of the steroid. Expand
Current status and bioanalytical challenges in the detection of unknown anabolic androgenic steroids in doping control analysis.
TLDR
The current situation in the detection of unknown AAS is reviewed and important steps have been made in order to minimize this analytical problem and different analytical strategies have been proposed, there are still some drawbacks related to each approach. Expand
Metabolism of methylstenbolone studied with human liver microsomes and the uPA⁺/⁺-SCID chimeric mouse model.
TLDR
A GC-MS/MS multiple reaction monitoring method was developed to detect methylstenbolone misuse and in one out of three samples, previously tested positive for methasterone, methylsten Bolone and U13 were additionally detected, indicating the applicability of the method. Expand
Enabling the inclusion of non-hydrolysed sulfated long term anabolic steroid metabolites in a screening for doping substances by means of gas chromatography quadrupole time-of-flight mass spectrometry.
TLDR
This work describes for the first time a validated ITP that allows the detection of non-hydrolysed sulfated metabolites of AAS while, simultaneously, remaining capable of detecting a vast range of other classes of compounds, as well as the quantification of endogenous steroids, as required for an ITP compliant with the applicable WADA regulations. Expand
Advances in the detection of designer steroids in anti-doping.
TLDR
The structural modifications of the already known AAS to create new designer molecules are explained and progress in the detection of designer AAS using mass spectrometry and bioassays; analytical data processing of the unknown AAS; metabolite synthesis; and, long-term storage of urine and blood samples are discussed. Expand
Drug-drug interaction and doping: Effect of non-prohibited drugs on the urinary excretion profile of methandienone.
TLDR
The results from the in vitro and in vivo experiments showed that the formation of the hydroxylated and dehydrogenated metabolites was significantly reduced in the presence of itraconazole, ketoconazoles, miconazole and nefazodone, whereas the production of the 18-nor-hydroxylation metabolites and glucuronidation reactions was reduced significantly only in the absence of ketoconAZole and miconzole. Expand
Statistical analysis of fragmentation patterns of electron ionization mass spectra of enolized-trimethylsilylated anabolic androgenic steroids
Abstract Anabolic androgenic steroids (AAS) are included in the List of prohibited substances of the World Anti-Doping Agency (WADA) as substances abused to enhance athletic performance. Gas… Expand
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