Scatter factor is a fibroblast-derived modulator of epithelial cell mobility

  title={Scatter factor is a fibroblast-derived modulator of epithelial cell mobility},
  author={Michael G. P. Stoker and Ermanno Gherardi and M B Perryman and Julia Gray},
Various factors are known to regulate cell growth and differentiation, but less is known of agents which affect movement and positioning, particularly in epithelial-mesenchymal interactions. Cultured human embryo fibroblasts release a protein with a relative molecular mass (M r) of ∼50,000 (50K) that affects epithelial cells by causing a disruption of junctions, an increase in local motility and a scattering of contiguous sheets of cells. To investigate specificity, a range of cells has been… 
Effect of scatter factor on motility of epithelial cells and fibroblasts
  • M. Stoker
  • Biology
    Journal of cellular physiology
  • 1989
The results confirm the cell specificity and paracrine role of the scatter factor and show that this fibroblast‐derived molecule can directly stimulate the movement of epithelial cells.
Protein factors which regulate cell motility
SF, AMF, and MSF are soluble and heat labile proteins with Mr of 77, 55, and 70 kd by SDS-PAGE, respectively, and may be members of a new class of cell-specific regulators of motility.
Down-regulation of scatter factor in MRC 5 fibroblasts by epithelial-derived cells. A model for scatter factor modulation.
It is concluded that inhibition of the SF activity expressed by MRC 5 cells by co-culture with SVK14 involves transcriptional regulation.
Scatter factor: molecular characteristics and effect on the invasiveness of epithelial cells
Purified scatter factor in fact induces the invasiveness into collagen matrices of MDCK epithelial cells, and induces or promotes theinvasiveness of a number of human carcinoma cell lines.
Hepatocyte growth factor/Scatter factor in the eye
Cell-scatter assay.
The method described in this chapter is intended specifically for measuring the scatter response of Madin-Darby canine kidney cells to hepatocyte growth factor stimulation.
Smooth muscle releases an epithelial cell scatter factor which binds to heparin
Vascular smooth muscle cells produce an epithelial cell scattering factor with properties similar to the fibroblast-produced factor, including a high affinity for heparin, which is potentially important because they may represent a new class of proteins that primarily regulate cell mobility rather than growth and differentiation.
Human Lung Cancer Cell Line Producing Hepatocyte Growth Factor/Scatter Factor
Two human lung cancer cell lines, PC‐1 and PC‐13, were found to produce a protein which was indistinguishable from HGF/SF with regard to biological activities and immunological characteristics, although they were derived from epithelial cells.
Rearrangements of desmosomal and cytoskeletal proteins during the transition from epithelial to fibroblastoid organization in cultured rat bladder carcinoma cells
It is demonstrated that the modulation of desmosomal proteins, specifically an increase in soluble desmoplakins, is a relatively early event in cell dissociation and in epithelial- mesenchymal transition, and the value of this experimental system is discussed as a physiologically relevant approach for studying the regulation of the assembly and disassembly of Desmosomes and other intercellular adhesion structures.
Scatter factor and hepatocyte growth factor: activities, properties, and mechanism.
  • M. Bhargava, A. Joseph, R. Zarnegar
  • Biology, Medicine
    Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
  • 1992
The findings suggest that by structural, functional, immunological, and mechanistic criteria, human SF and human HGF are essentially identical.