Scanning the cell surface proteome of cancer cells and identification of metastasis-associated proteins using a subtractive immunization strategy.

@article{Rasmussen2009ScanningTC,
  title={Scanning the cell surface proteome of cancer cells and identification of metastasis-associated proteins using a subtractive immunization strategy.},
  author={Nicolaj Rasmussen and Henrik J. Ditzel},
  journal={Journal of proteome research},
  year={2009},
  volume={8 11},
  pages={5048-59}
}
Identification of the cell surface proteome and comparison of their expression between cells with different phenotypic characteristics is crucial to the discovery of novel cancer drug targets as well as elucidating the basic biologic processes of cancer. However, cell surface proteomics are complex and technologically challenging, and no ideal method is currently available. Here, we describe a strategy that allows scanning of the entire cell surface and identification of molecules that exhibit… CONTINUE READING